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An electrochemical platform for studying biomembrane processes   Lucia Becucci and Rolando Guidelli

An electrochemical platform for studying biomembrane processes

128 страниц. 2014 год.
LAP Lambert Academic Publishing
Tethered bilayer lipid membranes, tBLMs, are biomimetic membranes consisting of a lipid bilayer interposed between the aqueous solution and a hydrophilic chain, called spacer, anchored to a metal electrode, which are used to incorporate single peptides or membrane proteins, so as to elucidate their structure-function relationships. Hg is a particularly convenient supporting electrode material for tBLMs thanks to its liquid state, which imparts to the lipid bilayer a fluidity and lateral mobility comparable with that of biological membranes, but with a much higher robustness and resistance to electric fields. The free movement of lipid molecules enables mercury-supported tBLMs to react to the presence of proteins, charges and physical forces in a dynamic and responsive manner, mimicking the functionality of living cell membranes. This review describes the way in which the structure of these tBLMs is affected by the incorporation and functional activity of peptides and small proteins...
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