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Antioxidant, Antimicrobial and Toxicological Investigation of Propolis   Md. Yousuf Ali and Utpal Kumar Mondal

Antioxidant, Antimicrobial and Toxicological Investigation of Propolis

92 страниц. 2012 год.
LAP Lambert Academic Publishing
Propolis was extracted with methanol and the concentrated extract was then partitioned with pet-ether,carbon tetrachloride and chloroform and ethyl acetate.The Ethyl acetate extract of Propolis were subjected to free radical scavenging activity by the method of Brand-Williams et al., 1995. Here, tert-butyl-1-hydroxytoluene (BHT) was used as reference standard. In this investigation Propolis showed moderate free radical scavenging activity with the IC50 value of 220 ?g/ml.The Ethyl acetate extract of propolis were subjected to antimicrobial screening at a dose 400 ?g/disc. The crude extract showed mild antimicrobial activity with average zone of inhibition 7-8 mm.The extract was further subjected to brine shrimp lethality test.It showed cytotoxicity with a considerable potency. The LC50 value of the ethyl acetate extract of propolis was found to be 1.089 ?g/ml.
 
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