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Polarity and temporal regulation in bacteriophage Lambda   Ekaterina Sodolevsky

Polarity and temporal regulation in bacteriophage Lambda

116 страниц. 2013 год.
LAP Lambert Academic Publishing
The Q protein of bacteriophage lambda mediates antitermination in the pR? operon and is required for the expression of the late genes of the bacteriophage. Termination at the tR? terminator is eliminated in the presence of Q. There are conflicting reports about the action of the Q protein downstream: some investigations show that Q can override terminators while others show that nonsense mutations can lead to polarity, albeit “localized”. This polarity seems to differ from the classical type because just one or several adjacent genes are affected, but not those further downstream. In the present investigation, it was shown that besides the pR? promoter there are no other promoters within the pR? operon and all the late genes are transcribed as a single polycistronic mRNA. An amber mutation at the start of the D gene leads to classical genetic polarity that affects all late genes downstream. At the same time, the results show that the morphogenetic genes seem to be organized in...
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