Добро пожаловать в клуб

Показать / Спрятать  Домой  Новости Статьи Файлы Форум Web ссылки F.A.Q. Логобург    Показать / Спрятать

       
Поиск   
Главное меню
ДомойНовостиСтатьиПостановка звуковФайлыДефектологияКнижный мирФорумСловарьРассылкаКаталог ссылокРейтинг пользователейЧаВо(FAQ)КонкурсWeb магазинШкольникамКарта сайта

Поздравляем!
Поздравляем нового Логобуржца Monita со вступлением в клуб!

Реклама

КНИЖНЫЙ МИР

Cloning and expression of recombinant Fasciola antigen?   Nahed Hussien

Cloning and expression of recombinant Fasciola antigen?

120 страниц. 2012 год.
LAP Lambert Academic Publishing
Fasciolosis is a liver disease caused by Fasciola hepatica or F. gigantica that causes significant economic loss in cattles, other animal species and man. The appearance of Fasciola hepatica populations that are resistant to common flukicidal drugs make a need for the development of anti-liver fluke vaccines. Though we selected a target Fasciola gene that expresses (rFhp) protein, in which its native form shows significant induction to lymphoproliferative response of Peripheral blood mononuclear cells. Then we amplified the gene through Polymerase chain reaction process using ?gt11 specific primers. This gene was cloned through TOPO (TA) cloning vector in XL10-Gold competent cells, then positive colonies that contain rTOPO were used for the excision of the gene to be expressed in expression vectors. ECORI digestions of the purified plasmids were done to detect the existence of the target insert. The results showed the excision of Fh?400 from recombinant PQE32 vector and ...
 
- Генерация страницы: 0.04 секунд -