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Engineering Hexuronic Acid Catabolism   Hugo Dantas

Engineering Hexuronic Acid Catabolism

68 страниц. 2014 год.
LAP Lambert Academic Publishing
Metabolic engineering targets for the production of high-value compounds. Citrus peel is produced ~15,000,000 t per year worldwide causing environmental problems. Its main constituent is D-galacturonic acid and can be converted to useful chemicals using genetically engineered strains. Engineered Aspergillus niger were used in solid and submerged state fermentation to convert orange peel to L-galactonate in a consolidate process. The gaaB coding for L-galactonate dehydrogenase was deleted (?gaaB) and ii) the gaaB was deleted and the gaaA coding for D-galacturonate reductase was overexpressed (?gaaB-gaaA). ?gaaB was able to convert up to 87 % of D-galacturonic acid to L-galactonate. Also, in this work, the eukaryotic D-Glucuronic acid pathway was studied. A decarboxylase that converts 3-keto-L-gulonate to L-xylulose remains poorly characterized and the gene is not known. A coupled enzyme assay was used to detect its activity. In the assay, ammonium sulfate precipitates from bovine...
 
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