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Study on grapevine die-back in Basrah southern Iraq   Mohanad Khalaf Mohammed Ameen and Abdullah H.Al-Saadoon

Study on grapevine die-back in Basrah southern Iraq

52 страниц. 2013 год.
LAP Lambert Academic Publishing
In Basrah,grapevines suffer from dieback.Lasiodiplodia theobromae and Neoscytalidium dimidiatum were isolated from diseased grapevinesVitis vinifera L.and identified based on morphological characteristics and DNA sequence data of the rDNA internal transcribed spacer (ITS)region . The results of the pathogenicity test conducted under greenhouse conditions for L.theobromae and N.dimidiatum revealed that both species were the causal agents of grapevines diebacks in Basrah, Southern Iraq.A brief description is provided for the isolated species.Histopathological changes of 2-years old grapevine cv.Abbassi inoculated with Lasiodiplodia theobromae(syn.Botryodiplodia theobromae)were studied. Samples were taken at 7 and 25 days after artificial inoculation.At 7 days,cross and longitudinal sections revealed the presence of disorganized cells,degenerated areas and vascular bundles.The intra–and intercellular invasion of the inoculated tissues by the fungus were evident with dark brown color.At...
 
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