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Isolation of Mycobacterium tuberculosis   Priya Mehrishi,Shinu Pottathil and Varsha A. Singh

Isolation of Mycobacterium tuberculosis

84 страниц. 2014 год.
LAP Lambert Academic Publishing
The study was designed to compare the ef?cacy of cetylpyridinium chloride (CPC) and sodium chloride (NaCl) decontamination method with N-acetyl L-Cystine (NALC) and sodium hydroxide (NaOH) decontamination (the reference method) method for the recovery of Mycobacterium tuberculosis (MTB) from clinically suspected cases of pulmonary tuberculosis. To evaluate CPC-NaCl and NALC-NaOH decontamination methods, sputum specimens (n = 100) were studied (culturing on Lowenstein-Jensen medium), and the performances were compared. The CPC-NaCl decontamination method demonstrated a sensitivity, speci?city, negative predictive value, and positive predictive value of 100%, 87.63%, 20%, and 100%, respectively, when compared to NALC-NaOH decontamination method. In summary, CPC-NaCl decontamination method effectively detected signi?cantly higher number of MTB cases (n = 15) than NALC-NaOH decontamination method (n = 3) particularly in sputum with scanty bacilli and smear-negative cases, indicating the...
 
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