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NS1 Antigen ELISA & RT-PCR in Acute Phase of Japanese Encephalitis   Rajesh Kumar Verma,T. N. Dhole and A. Ayyagari

NS1 Antigen ELISA & RT-PCR in Acute Phase of Japanese Encephalitis

132 страниц. 2012 год.
LAP Lambert Academic Publishing
India, where drought and floods occur periodically or with regular frequency, the occurrence of JE can be forecast. Large water pools ideal for breeding of vectors of JE are conducive for nesting and breeding of Ardeid birds such as egrets and herons. One can therefore predict with reasonable accuracy an impending epidemic. In fact, research on methods of predicting an epidemic should be a continuous process. Instead the authorities prefer to carry out fire-fighting operations during epidemics. Commercial kits are now available for the detection of JE NS1 antigen, IgM antibodies using capture ELISA. These tests can be readily performed and very helpful. PCR tests have been developed and have a role to play in JE diagnosis. PCR requires facilities not routinely available in peripheral labs. The short period of viraemia in cases may also limit the usefulness of PCR as a clinical diagnostic tool. Viral isolation can be performed, but it is slow and technically difficult, and is often...
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